The Thermal Cycler (also known as a Thermocycler, PCR Machine, or DNA Amplifier) is a laboratory device that uses the Polymerase Chain Reaction to amplify DNA segments (PCR). A thermal block with holes allows tubes containing PCR reaction mixtures to be inserted into the device. The cycler then takes separate, pre-programmed steps to boost and lower the block's temperature. The polymerase chain reaction, or PCR, is a popular DNA-replication technique that allows researchers and technicians to replicate a single DNA sample millions of times. This, of course, aids in the accurate analysis and study of specific DNA segments. The technique was created in the 1980s, but it has already led to a slew of research achievements in that short period. Thermal cycling, a heating and cooling process that provides the conditions for DNA replication, is used to carry out the actual PCR process. Denaturing, annealing, and expanding are the three stages of PCR thermal cycling, which are explained below: Denaturing: Denaturing is the first step in the PCR thermal cycling process. This heats the DNA sample to the point that the double-stranded sample separates into two separate DNA strands. Annealing: After the initial denaturing heating, the temperature is lowered to allow the DNA primers to bind to the sample DNA through annealing. Extending: It entails an additional temperature rise. The Taq polymerase enzyme is able to replicate the template DNA after this final temperature rise. Real-time quantitative PCR (qPCR) machines are still very massive, but they track the reaction's progress in real time, removing the need for gel electrophoresis and UV gel documentation or imaging. Those who are interested to submit their manuscript in our journal for publication, the can submit it either online through given link: https://www.longdom.org/submissions/clinical-chemistry-laboratory-medicine.html or send it to us as an email attachment to below given mail id.

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