Laboratory Instruments: Compound microscope


When a tiny object is positioned just outside the focus of a compound microscope's objective lens, a simulated, inverted, and highly magnified image of the object is created at the shortest distance of distinct vision from the eye kept close to the eye piece. A compound microscope has two convex lenses: a small aperture objective lens O and a wide aperture eye piece E. The objective lens is the one that is aimed at the object, while the eye piece is the one that is aimed at our eyes. These two convex lenses, the focus and the eye portion, have a short focal length and are attached to the free ends of two sliding tubes at an appropriate distance. While both the objective lens and the eye piece have a short focal length, the objective lens O has a slightly shorter focal length than the eye piece E.

To determine the total magnification when viewing an image with a compound light microscope, multiply the objective lens' power, which is usually 4x, 10x, or 40x, by the eyepiece's power, which is typically 10x. As a result, combining a 10x eyepiece with a 40X objective lens yields a magnification of 400X. The specimen can now be seen at 400 times magnification with the naked eye, revealing microscopic images.

In pathology laboratories, a compound microscope is very useful for identifying diseases. Human cells are extracted and examined under the microscope in forensic laboratories to identify and solve various crime cases. Compound microscopes may detect the presence or absence of minerals, as well as the presence or absence of metals. The use of a microscope for conducting academic experiments benefits students in schools and colleges. It allows you to see and appreciate the microbial world of bacteria and viruses, which is otherwise hidden from view. A compound microscope is used to study plant cells and determine the microorganisms that thrive on them. A compound microscope has thus proven to be important for biologists.

Since the picture is created in a brightly illuminated area, the popular light microscope is also known as a bright field microscope. Since the specimen or object is denser and more opaque than the surroundings, the picture appears darker. A portion of the light that passes through or through an object is absorbed. For examination of preserved and stained material as well as live and unstained object or material, a bright field microscope is used. In the case of live unstained specimens, however, differentiation is low. Dark field, phase contrast, and differential interference contract microscopes are among the special microscopes used to examine them.

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Journal of Clinical chemistry and Laboratory Medicine